|
KMID : 1005220100250010015
|
|
Journal of Embryo Transfer
2010 Volume.25 No. 1 p.15 ~ p.20
|
|
|
Effect of OPU (Ovum Pick-Up) Duration on the Rate of Collected Ova and In Vitro Produced Blastocyst Formation
|
|
Jin Jong-In
Kwon Tae-Hyeon
Choi Byeong-Hyun
Kim Sung-Soo
Jo Hyun-Tae
Kong Il-Keun
|
|
|
Abstract
|
|
|
|
This study was performed to identify the optimal timing for oocyte donor replacement during OPU procedure. OPU was carried out to collect oocytes from every donor at an interval of days (2 times a week). The collected oocytes were matured in vitro in TCM-199 supplemented with 10% FBS, 10 mg/ml of FSH and 1 mg/ml of estradiol for 24 h. After 24 h of exposure to sperm, the presumptive zygotes were cultured in CR1aa medium supplemented with 4 mg/ml of BSA for 3 days before being changed to CR1aa medium with 10% of FBS for another days. The mean numbers of retrieved oocytes were remained constantly up to 3 months (, , ), but significantly decreased at over 4 to 6 months (, , ) (p<0.05). The blastocyst development potential was also very similar rate from 1 to 3 months (37.2%, 40.4% and 44.6%), but significantly decreased from 4 to 6 months (24.8%, 29.3% and 28.6%, respectively) (p<0.05). The production of OPU derived embryos in periods of 1 to 3 months (, and ) were significantly higher than those in 4 to 6 months (, and , respectively) (p<0.05). In conclusion, the efficient periods for the production of OPU derived embryos was until 4 months, twice per week to produce over 64 transferable embryos and then replace new donor after 3 months use. The best replacement time is 3 months and could be maximized production of OPU derived embryos.
|
|
|
KEYWORD
|
|
|
OPU, duration, bovine, oocyte, blastocyst
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|